Western blot analysis of extracts from HeLa cells, untransfected or transfected with SignalSilence® eIF4B siRNA #6416, using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 uM) as indicated.
Western blot analysis of extracts from HeLa cells, transfected with either nonspecific control siRNA or eIF4B siRNA. eIF4B was detected using eIF4B Antibody #3592, and eIF4E was detected using eIF4E Antibody #9742. The eIF4B Antibody confirms silencing of eIF4B expression, and the eIF4E Antibody is used to control for loading and siRNA specificity.
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.